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1 BIODEGRADATION OF HYDROCARBONS IN WATER
1.1 Biodegradation by Hegsurf.dc
of SAE 30 oil and diesel in water
| A 50:50 mixture of SAE 30 oil and diesel was added to 10 litres
of water in a concentration of 500 mg/L. One sample was treated
with Hegsurf.dc, the other was kept as control. The residence
time was four hours during which time 82% degradation was achieved,
compared with 39% in the control. |

1.2 Biodegradation by Hegsurf.dc
of components of diesel fuel spilled in water
| The "Diesel Aquarium Test": Percentages shown are of the original
concentration of each component, after treatment, with aeration,
for 24 hrs. The untreated control showed appreciable degradation
of only two components - naphthalene and anthracene. |

The component hydrocarbons are:
| 1. Napthalene |
5. Phenanthrene |
9. Benzo (a) anthracene |
13. Benzo (a) pyrene |
| 2. Acenapthalene |
6. Anthracene |
10. Chrysene |
14. Dibenzo (a,h) anthracene |
| 3. Acenapthene |
7. Fluoranthene |
11. Benzo (b) fluoranthene |
15. Benzo (g,h,i) perydene |
| 4. Fluorine |
8. Pyrene |
12. Benzo (k) fluoranthene |
16. Ideno (1,2,3-CD) pyrene |
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2 BIODEGRADATION OF HYDROCARBON WASTES
2.1 Biodegradation of used motor oil by Hegsurf.pw
| One pint of used motor oil was treated with 2 gallons of a
mixture of one part Hegsurf.pw diluted with four parts water.
A circulating pump was installed in the drum and was run during
the daytime only. No filter was used to remove sludge. After
25 days, 92% degradation had been achieved. |

2.2 Biodegradation by Hegsurf.dc
of diesel and gasoline run-off from concrete surfaces
| The test examined the effect over one month of Hegsurf.dc
applied to collected run off from spills of gasoline and diesel
on concrete. Rapid and very large reductions in TPH levels were
achieved, associated with a marked increase in total bacterial
heterotrophs and in hydrocarbon degraders (as defined by their
ability to use oil or phenanthrene/toluene as a sole carbon
source). |

2.3 Biodegradation by Hegrem
of PCBs in transformer oil
| Transformer oil containing PCB 1242 and PCB 1260 was treated
with Hegrem until fully encapsulated and dry to the touch. Water
was then added to give a 30% moisture content. After 35 days,
degradation of 45% and 55% had been achieved; after a further
35 days, degradation was respectively 82% and 84%. |

2.4 Biodegradation/composting of motor oil
by Hegrem and Hegsorb
| 30W HD motor oil was absorbed by a mixture of Hegrem and Hegsorb
until dry to touch. The mixture was then kept wet and turned
every two days. After 105 days, 92% degradation had been achieved. |

2.5 Biodegradation of parts washer sludge
by Hegrem
| Half the sludge from a heavy duty parts washer was treated
with Hegrem, with half the sample being untreated as a control.
Initial contamination of 500,000 ppm TPH was reduced to 57,000
ppm after 65 days. Temperature was then dropped for 85 days,
suspending biodegradation. Temperature and thus biodegradation
were then restored until after a further 100 days TPH were reduced
to 4,835 ppm (99% bioremediation). The control after 250 days
showed biodegradation of only 8%. |

3 BIOREMEDIATION OF CONTAMINATED
LAND
3.1 Bioremediation by Hegrem
of land contaminated by spilled diesel
| Hegrem was applied to the contaminated land, tilled and moistened.
The original contamination of c. 150,000 ppm (average from samples
taken) was reduced after 12 days to an average of c. 55,000
ppm. This is a radical reduction over what is a very short period
of time by the standards of bioremediation work. Percentages
are residue as proportion of original. |

3.2 Power Station land contaminated by diesel
bioremediated by Hegrem
| A 1.5 acre site contaminated at levels between 600 and 3,400
ppm of diesel was treated with Hegrem and readings were taken
at 8 locations after 34 and 61 days. All locations showed bioremediation
to less than 100 ppm after two months. |

3.3 Hegrem's
effectiveness in reducing hydrocarbon pollution in soil
| 50 cubic yards of soil contaminated by 44 - 49,000 ppm TPH
were treated with 8,000 lbs of Hegrem, with initial tilling
and water added, but no subsequent aeration. After two months,
contamination had been reduced to local compliance levels. |

3.4 Biodegradation by Hegrem
of crude oil in contaminated land
| Land contaminated by crude oil to an average level of 387,000
ppm was treated with Hegrem until contamination was reduced
to 124 ppm (99.97% degradation) after 160 days. |

4 FIRE RETARDATION
REDUCTION OF VOLATLITY & FLAMMABILITY OF HYDROCARBONS AFTER
APPLICATION OF HEGREM (or
HEGFLOOR) and HEGSURF.dc
4.1 Reduction of flammability and increase
in burn time of gasoline when treated with Hegrem
or Hegfloor
| 100 ml of Hegfloor/Hegrem was added to 50 ml of gasoline,
and was left to absorb it for increasing periods before ignition.
The longer the period, the slower the gasoline burned. When
the amount of Hegfloor/Hegrem used was doubled, the gasoline
would not burn at all. |

4.2 Reduction by Hegsurf.dc
of Volatile Organic Compounds (VOC) after a spill of gasoline on
concrete
| One part of a 50:50 mixture of Hegsurf.dc and water was applied
to one part of gasoline spilled on concrete. The results of
this and of a test with a control sample which was untreated
were measured by a Scott Alert Explocymeter in a sealed container
1/4 inch above the surface. |

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